megatomi.com for Dummies
megatomi.com for Dummies
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Our new Obvious+ tissue clearing approach is the only real strategy that delipidates samples with no transform in morphology and with minimum influence on structural integrity.
Megatome is usually a vibrating microtome meant to portion a wide variety of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With substantial blade vibrating frequency and minimized blade deflection, Megatome permits significant-throughput tissue sectioning with uniform floor profile, in addition to nominal tissue problems and data loss.
Antibodies may well acquire months to diffuse by only some millimeters of tissue, that has a steep labeling gradient from area to Main.
Megatome is created for precision: the blade vibrates at an increased frequency and bigger amplitude selection than other microtomes, and incorporates a exclusive deflection Management mechanism.
SE uses a rotational electric powered industry to disperse remarkably electromobile molecules (for example antibodies or surfactant micelles) through a porous sample without having harmful electrically charged buildings in the tissue. This permits 2-4 working day clearing of intact organs,
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Megatome is usually a novel microtome that permits for high-precision sectioning of a wide array of tissue samples – from organoids, to arrays of animal organs, to intact human brain hemispheres – with negligible tissue hurt and information reduction.
eFLASH is really a immediate tissue megatomi.com labeling strategy that allows for uniform total-organ staining in 20 rounds of labeling.
The SmartSPIM mild sheet microscope presents unparalleled resolution and acquisition pace all over intact tissue volumes, even though its modular design means that you can upgrade and personalize individual parts.
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Both down load a release or produce a distribution zip as outlined earlier mentioned. Unzip the archive to a wanted spot.
Leverage the Crystal clear+ tissue clearing system, together with eFLASH and patented stochastic electrotransport technologies, to speedily crystal clear and label full organs. Important highlights and functions incorporate:
Our novel Protect tissue preservation system forms intramolecular bonds making use of polyfunctional, versatile epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.